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KMID : 0545120180280122029
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 12 p.2029 ~ p.2035
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Biochemical Characterization of Alkaliphilic Cyclodextran Glucanotransferase from an Alkaliphilic Bacterium, Paenibacillus daejeonensis
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Yang So-Jin
Ko Jin-A
Kim Hae-Soo
Jo Min-Ho
Lee Ha-Nul
Park Bo-Ram
Kim Young-Min
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Abstract
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Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a single protein band of 111 kDa. PDCITase showed optimum activity at pH 8.0 and retained 100% of activity within a broad pH range (7.0?11.5) after 18 h, indicating alkaliphilic or alkalistable CITase properties. In addition, PDCITase produced CI-7 to CI-17, CI-18, and CI-19, which are relatively large cycloisomaltooligosaccharides yet to be reported. Therefore, these large cycloisomaltooligosaccharides can be applied to the improvement of water solubility of pharmaceutical biomaterials.
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KEYWORD
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Cyclodextran, cycloisomaltooligosaccharide glucanotransferase, Paenibacillus daejeonensis, glycoside hydrolase family 66
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